Rhodes has been famous since antiquity as the site of Colossus of Rhodes, one of the Seven Wonders of the Ancient World. The citadel of Rhodes, built by the Hospitalliers, is one of the best-preserved medieval towns in Europe, which in 1988 was designated as a UNESCO World Heritage Site.

Today, the city of Rhodes is an important Greek urban center and popular international tourist destination.

The city of Rhodes was formed by the cities of Ialyssos, Kamiros and Lindos in 408 BC, and prospered for three centuries during its Golden Age, when sea trade, skilled shipbuilders, and open-minded politicians of the city kept it prosperous until Roman times. The Colossus of Rhodes, one of the original Seven Wonders of the Ancient World was built by Chares of Lindos between 304 and 293 BC, which took 12 years and was completed in 282 BC. The statue represented their sun god Helios, which stood at the harbor entrance. The ancient city had a well-constructed sewage system as well as a water supply network as designed by Hippodamus. A strong earthquake hit Rhodes about 226 BC, badly damaging the city and toppling the Colossus. The Knights Hospitallers captured and established their headquarters on Rhodes when they left Cyprus after the persecution of the Knights Templar in 1307. Pope Clement V confirmed the Hospitallers possession of the Island in 1309. The Knights remained on the Island for the next two centuries.

In 1444, the Mamluk fleet of Egypt laid a siege to Rhodes, but the Knights aided by the Burgundian naval commander Geoffroy de Thoisy beat off the Muslim attack.

After the Fall of Constantinople in 1453 the Ottoman Empire began a rapid expansion and in 1480 Sultan Mehmet launched an invasion of Rhodes commanded by Mesic Pasha. The defenders repelled Turkish attacks from both landward and seaward sides and the invaders left the Island in defeat. The defeat halted a concurrent invasion of the Italian peninsula by Ottoman forces and prevented possible Muslim incursion and control of Western Europe.

The British bombs that fell on the medieval city of Rhodes in 1944 claimed lives and destroyed a great number of buildings, leaving large gaps in the urban tissue. One of the first decrees of the Greek administration designated those areas as reserved for future excavations and a number of edifices as safeguarded buildings. In July 1944 the Nazis ordered the deportation of over 1,700 Jews of Rhodes including men, women, and children, of whom 1,200 were murdered at Auschwitz.

In 1957, a new city plan was approved by a decree and in 1960 the entire medieval town was designated as a protected monument by the Ministry of Culture. In 1961 and 1963 new decrees were issued concerning the new city plan. They provided for the widening of existing streets and the opening of new ones. However, these were not implemented in the old city due to the resistance of the Archaeological Service. In 1988, the old town of Rhodes was designated as a World Heritage City by UNESCO.